Our group studies the host biological functions involved in the replication and pathogenesis of human viruses and in the response to these infections.
In particular, we are exploring the relationship between HIV-1 (human immunodeficiency virus), the causative agent of AIDS, and the host cell in order to better understand the molecular mechanisms that control viral replication and spread, with the ultimate goal of identifying potential strategies to control or eradicate the virus.
– The laboratory deciphers the mechanisms of HIV-1 latency that lead to the persistence of cells harboring replication-competent but transcriptionally silent viruses. These reservoirs are established early in the course of infection and can persist for an extended period of time. If treatment is interrupted, viral rebound occurs rapidly.
– We are also exploring the molecular basis of HIV-1 expression at both transcriptional and post-transcriptional levels. The HIV-1 transcriptome is complex and must be finely regulated to allow the morphogenesis of infectious viral particles.
– In addition, the team is studying how HIV-1 counteracts the restriction factors, intrinsic barriers of the host to infection. Restriction factors are proteins with direct antiviral activity and are an important facet of the innate immune response controlling infection in a cell-intrinsic manner. HIV-1 has evolved countermeasures to evade these mechanisms. In particular, we are studying the restrictions that viruses face during the assembly and release of new virions.
The emergence of the highly pathogenic coronavirus SARS-CoV-2 and its rapid spread worldwide have recently led us to explore the host-virus interactions involved in the replication of this pathogen. Proteomic approaches are being developed to characterize the cellular factors essential for the morphogenesis of infectious SARS-Cov2 virions.
To reach these objectives, we use genetic, cell biology, molecular biology, imaging and virology techniques.
Top 5 publications
1- The viral restriction factor tetherin/BST2 tethers cytokinetic midbody remnants to the cell surface. Presle A, Frémont S, Salles A, Commere PH, Sassoon N, Berlioz-Torrent C, Gupta-Rossi N, Echard A. Curr Biol. 2021 May 24;31(10):2203-2213.e5.
2- Contribution of the Cytoplasmic Determinants of Vpu to the Expansion of Virus-Containing Compartments in HIV-1-Infected Macrophages. Leymarie O, Lepont L, Versapuech M, Judith D, Abelanet S, Janvier K, Berlioz-Torrent C. J Virol. 2019 May 15;93(11):e00020-19.
3- TIM-1 Ubiquitination Mediates Dengue Virus Entry. Dejarnac O, Hafirassou ML, Chazal M, Versapuech M, Gaillard J, Perera-Lecoin M, Umana-Diaz C, Bonnet-Madin L, Carnec X, Tinevez JY, Delaugerre C, Schwartz O, Roingeard P, Jouvenet N, Berlioz-Torrent C, Meertens L, Amara A. Cell Rep. 2018 May 8;23(6):1779-1793.
4- Characterization of E3 ligases involved in lysosomal sorting of the HIV-1 restriction factor BST2. Roy N, Pacini G, Berlioz-Torrent C, Janvier K. J Cell Sci. 2017 May 1;130(9):1596-1611
5- LC3C Contributes to Vpu-Mediated Antagonism of BST2/Tetherin Restriction on HIV-1 Release through a Non-canonical Autophagy Pathway. Madjo U, Leymarie O, Frémont S, Kuster A, Nehlich M, Gallois-Montbrun S, Janvier K, Berlioz-Torrent C. Cell Rep. 2016 Nov 22;17(9):2221-2233.
Dernières publications sur HAL :
- [hal-04279863] ATG5 selectively engages virus-tethered BST2/tetherin in an LC3C-associated pathwayby email@example.com (Delphine Judith) on 10 November 2023 at 17h14
Bone marrow stromal antigen 2 (BST2)/tetherin is a restriction factor that reduces HIV-1 dissemination by tethering virus at the cell surface. BST2 […]
- [hal-04136961] DNA topoisomerase 1 represses HIV-1 promoter activity through its interaction with a guanine quadruplex present in...by firstname.lastname@example.org (María José Lista) on 21 June 2023 at 23h00
Background: Once integrated in the genome of infected cells, HIV-1 provirus is transcribed by the cellular transcription machinery. This process is […]
- [inserm-03981639] Canonical and Non-Canonical Autophagy in HIV-1 Replication Cycleby email@example.com (Olivier Leymarie) on 9 February 2023 at 22h05
Autophagy is a lysosomal-dependent degradative process essential for maintaining cellular homeostasis, and is a key player in innate and adaptive […]
- [inserm-03981636] [Cellular and viral determinants control HIV-1 virus-containing compartment expansion in infected macrophages]by firstname.lastname@example.org (Leslie Lepont) on 9 February 2023 at 22h02
No abstract available