Biology of Intracellular Bacteria
Our major research interest is to understand how bacteria cause disease: what are the genetic factors conferring bacterial virulence, how do they evolve, what are the mechanisms by which they allow subverting host functions and more generally how do human pathogens emerge. We are useing Legionella as a model, as these bacteria are at the crossroad between an environmental bacterium (parasite of protozoa) and a human pathogen (replicating in alveolar macrophages). Legionella pneumophila and L. longbeachae, environmental bacteria parasitizing within free-living protozoa are also human pathogens causing a severe pneumonia that can be fatal if not treated promptly. Our projects are focused on deciphering (i) the host pathways that are manipulated during infection, (ii) the mechanisms that a bacterial pathogen may apply to establish infection and cause disease, and (iii) the evolution of a bacterium into an intracellular pathogen of amoeba and human macrophages. The biology to discover by studying host-Legionella interactions is fascinating as this bacterium is one of the best cell biologists or perhaps “a copycat eukaryote” that allows us to understand not only bacterial pathogenesis strategies but also to decipher host pathways that need to be subverted by a pathogen to cause disease.
Top 5 publications
1- Sahr T, Escoll P, Rusniok C, Bui S, Pehau-Arnaudet G, Lavieu G, Buchrieser C (2022) Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs to dampen the host immune response. Nature Communications, 13(1):762 doi: 10.1038/s41467-022-28454-x
2- Escoll P, Platon L, Dramé M, Sahr T, Schmidt S, Rusniok C, Buchrieser C (2021) Reverting the mode of action of the mitochondrial FOF1-ATPase by Legionella pneumophila preserves its replication niche. Elife.10:e71978.
3- Pérez-Cobas AE, Ginevra C, Rusniok C, Jarraud S, Buchrieser C (2020) Persistent Legionnaires’ Disease and Associated Antibiotic Treatment Engender a Highly Disturbed Pulmonary Microbiome Enriched in Opportunistic Microorganisms. mBio. 11(3):e00889-20. doi: 10.1128/mBio.00889-20.
4- Gomez-Valero L, Rusniok C, Carson D, Mondino S, Pérez-Cobas AE, Rolando M, Pasricha S, Reuter S, Demirtas J, Crumbach J, Descorps-Declere S, Hartland EL, Jarraud S, Dougan G, Schroeder GN, Frankel G, Buchrieser C. (2019) More than 18,000 effectors in the Legionella genus genome provide multiple, independent combinations for replication in human cells. Proc Natl Acad Sci U S A. 116(6):2265-2273.
5- Escoll P, Song OR, Viana F, Steiner B, Lagache T, Olivo-Marin JC, Impens F, Brodin P, Hilbi H, Buchrieser C. (2017) Legionella pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages. Cell Host Microbe. 22(3):302-316.e7
Dernières publications sur HAL :
- [pasteur-04143396] Hiding in the yolk: A unique feature of Legionella pneumophila infection of zebrafishby email@example.com (Flávia Viana) on 27 June 2023 at 15h58
The zebrafish has become a powerful model organism to study host-pathogen interactions. Here, we developed a zebrafish model to dissect the innate […]
- [pasteur-04132531] Legionella and mitochondria, an intriguing relationshipby firstname.lastname@example.org (Francisco Javier García-Rodríguez) on 19 June 2023 at 11h09
Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe pneumonia. L. pneumophila injects via a type-IV-secretion-system […]
- [pasteur-04123958] Bacterial methyltransferases: from targeting bacterial genomes to host epigeneticsby email@example.com (Monica Rolando) on 9 June 2023 at 15h05
Methyltransferase (MTases) enzymes transfer methyl groups particularly on proteins and nucleotides, thereby participating in controlling the […]
- [pasteur-03913556] The evolution and role of eukaryotic-like domains in environmental intracellular bacteria: the battle with a...by firstname.lastname@example.org (Jessica Martyn) on 27 December 2022 at 10h42
One sentence summary: This review aims to dissect the evolutionary processes that may lead to an enrichment of eukaryotic-like proteins in […]