Martin PICARD

Website: http://umr7099.ibpc.fr
ORCID: 0000-0002-6518-8900
Twitter: @BPM_IBPC

Our laboratory focuses on the study of the fundamental mechanisms of membrane protein systems, mainly in microorganisms but also in higher eukaryotes.

The laboratory’s projects are organized around 4 main themes:

1) energy coupling and study of supramolecular assemblies involved in the electron transfer chain.

2) Signaling mediated by G protein-coupled receptors (GPCRs).

3) Dynamics of membranes and membrane transport in bacteria.

4) Toolbox for the study of membrane proteins.

Our aim is to combine microbial physiology with biophysical (NMR, Stopped-Flow fluorimetry, …) or analytical methods (SEC MALLS, mass spectrometry, lipidomics …) together with structural biology (crystallography, electron microscopy) to access the atomic details, dynamics and function of membrane proteins at different time scales and spatial resolutions.

A second mission of the unit is to develop innovative methods in biochemistry, biophysics and synthetic biology to help rationalize and optimize the handling and study of membrane proteins (engineering of bacterial strains for the heterologous expression of membrane proteins, chemistry of amphipathic polymers, …).

Top 5 publications

Gagelin A, Largeau C, Masscheleyn S, Piel MS, Calderón-Mora D, Bouillaud F, Hénin J, Miroux B. 2023. Molecular determinants of inhibition of UCP1-mediated respiratory uncoupling. Nat Commun. 14(1):2594. https://doi.org/10.1038/s41467-023-38219-9

Pozza A, Giraud F, Cece Q, Casiraghi M, Point E, Damian M, Le Bon C, Moncoq K, Banères J-L, Lescop E, Catoire LJ. 2022. Exploration of the dynamic interplay between lipids and membrane proteins by hydrostatic pressure. Nat Commun. 13(1):1780. https://doi.org/10.1038/s41467-022-29410-5

Biou V, Adaixo RJD, Chami M, Coureux P-D, Laurent B, Enguéné VYN, De Amorim GC, Izadi-Pruneyre N, Malosse C, Chamot-Rooke J, et al. 2022. Structural and molecular determinants for the interaction of ExbB from Serratia marcescens and HasB, a TonB paralog. Commun Biol. 5(1):355. https://doi.org/10.1038/s42003-022-03306-y

Souabni H, Batista dos Santos W, Cece Q, Catoire LJ, Puvanendran D, Bavro VN, Picard M. 2021. Quantitative real-time analysis of the efflux by the MacAB-TolC tripartite efflux pump clarifies the role of ATP hydrolysis within mechanotransmission mechanism. Commun Biol. 4(1):493. https://doi.org/10.1038/s42003-021-01997-3

Marconnet A, Michon B, Le Bon C, Giusti F, Tribet C, Zoonens M. 2020. Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers. Biomacromolecules.:acs.biomac.0c00929. https://doi.org/10.1021/acs.biomac.0c00929

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