Website: https://www.iame-research.center/eq1/research-interests/
ORCID: 0000-0002-3796-1601
With the rise of antibiotic resistance and the emergence of new forms of virulence, it has become clear that microbial evolution is at the heart of infectious diseases. The aim of our team is to study the microbial adaptation with a quantitative methodology. For that purpose, we combine three approaches: experimental evolution, systems biology and population genetics/ comparative genomics.
Experimental evolution combined with whole genome sequencing allow us to uncover the molecular bases of adaptation in test tubes as well as in mice colonization models. Population genetics/Comparative genomics allow us to study rates and tempo of adaptation in the wild at different time scale: species wise, for a sequence type or within a host as commensal or pathogen and to compare the observed patterns with the ones derived from integrated models of adaptation, such as Fisher Geometric Model of Adaptation. Finally, we use systems biology approach, in which we study simulatenously thousands of mutants within small molecular systems (a gene, a small regularity network) to unravel the evolutionary and mechanistic constraints shaping the function of these molecular entities.
All these approaches take full benefit of the sequencing revolution, the computing progresses, as well as some high throughput phenotyping. Thanks to these technical advancements, we can increase both the level of replication and the description level of the clones and switch from a descriptive science to a quantitative one.
All the different facets of our expertise are used in the collaborative project DREAM, in which we will study the influence of human diet and host microbiota on the propagation of antibiotic resistance in our species of interest E. coli, using well characterized microbiota donors and artificial in vitro gut models.
2) We have a large expertise in molecular genetics, as well as sequence analysis from libraries of mutants to clones, populations as well as microbiota. We have an anaerobic hoot and a system allowing the maintenance of a microbiota over two weeks. A robotic platform of fluidic (TECAN EVOLUTION) and TECAN plate readers.
Top 5 publications
- Vigué, G. Croce ,M. Petitjean,E. Ruppé, O. Tenaillon*, M. Weigt*: Deciphering polymorphism in 61,157 Escherichia coli genomes via epistatic sequence landscapes. Nature Communication, vol 13(1):4030, 2022. (*contribution egale)
- Choudhury, J.A. Fenster, R.G. Fankhauser, J.L. Kaar, O. Tenaillon*, and R.T. Gill*: “CRISPR/Cas9 recombineering-mediated deep mutational scanning of essential genes in Escherichia coli.”Molecular Systems Bioliogyvol. 16, no. 3, pp. e9265, 2020. (*contribution egale)
- Kemble, C. Eisenhauer, A. Couce, A. Chapron, M. Magnan, G. Gautier, H.L. Nagard, P. Nghe, and O. Tenaillon: “Flux, toxicity, and expression costs generate complex genetic interactions in a metabolic pathway.”Science Advancesvol. 6, no. 23, pp. eabb2236, 2020.
- Lescat, A. Launay, M. Ghalayini, M. Magnan, J. Glodt, C. Pintard, S. Dion, E. Denamur, and O. Tenaillon: “Using long-term experimental evolution to uncover the patterns and determinants of molecular evolution of an Escherichia coli natural isolate in the streptomycin-treated mouse gut.”Molecular EcologyVol. 26 no. 7, pp 1082-1817, 2017.
- Couce, L.V. Caudwell, C. Feinauer, T. Hindré, J.-P. Feugeas, M. Weigt, R.E. Lenski, D. Schneider, and O. Tenaillon: “Mutator genomes decay, despite sustained fitness gains, in a long-term experiment with bacteria.”Proc. Natl. Acad. Sci. vol. 114, no. 43, pp. E9026–E9035, 2017
Dernières publications sur HAL :
- [hal-04420996] Impact of concurrent training (HIIT + RT) on body composition and gut microbiota in postmenopausal women with...by ano.nymous@ccsd.cnrs.fr.invalid (Marine Dupuit) on 27 January 2024 at 10h30
This communication is an oral presentation about a published paper entitled " Impact of concurrent training (HIIT + RT) on body composition and gut […]
- [hal-03774131] A 90-day Oral Exposure to Food-grade Gold at Relevant Human Doses Impacts the Gut Microbiota and the Local Immune...by ano.nymous@ccsd.cnrs.fr.invalid (Lauris Evariste) on 11 January 2024 at 14h52
Background: Edible gold (Au) is commonly used as a food additive (E175 in EU) for confectionery and cake decorations, coatings and in beverages. […]
- [hal-03775382] Effets d’une exposition alimentaire, périnatale et chronique alimentaire, à des doses réglementaires de...by ano.nymous@ccsd.cnrs.fr.invalid (Lorraine Smith) on 12 September 2022 at 14h55
Contexte Le glyphosate est l’herbicide le plus utilisé au monde. L’exposition des consommateurs au glyphosate est principalement alimentaire et […]
- [hal-03775223] Mice exposed to food-grade titanium dioxide from in utero life to adulthood show sex-specific gut microbiota and...by ano.nymous@ccsd.cnrs.fr.invalid (Lauris Evariste) on 12 September 2022 at 13h58
Background: Food additives are one major hallmark of ultra-processed food in the Western diet, a food habit often associated with metabolic […]