Website: https://research.pasteur.fr/en/team/cellular-biology-of-microbial-infection/
ORCID: 0000-0002-7481-4846
The goal of the work of the lab is to gain knowledge on the normal behavior of the eukaryotic cell, and on its disruptions during an infectious process. We focus on the mechanisms of entry and intracellular fate of intracellular bacteria, Chlamydia. Chlamydiae species pathogenic to humans, mainly Chlamydia trachomatis and Chlamydia pneumoniae, cause a number of diseases, including trachoma, pelvic inflammatory disease and pneumonia. Primary infections are often minor or asymptomatic; the consequences appear long after infection. Throughout their cycle in the host cell, chlamydiae remain in a membrane-bound compartment called an inclusion. We investigate the interactions between the bacteria and the cells during infection. We focus mainly on the functional study of proteins secreted by the bacteria into the host cytoplasm by a process called type III secretion. Recent themes of study include the interplay between the metabolism of the host and that of the pathogen, as well as the consequence of the infection on the epigenome of the host.
Top 5 publications
- Triboulet S, N’Gadjaga M.D., Niragire B., Köstlbacher S., Horn M., Aimanianda V. and Subtil A. (2022) CT295 Is Chlamydia trachomatis’ Phosphoglucomutase and a Type 3 Secretion Substrate. Front Cell Inf Microbiol 12. doi 10.3389/fcimb.2022.866729
- Tang C, Liu C, Maffei B, Niragire B, Cohen H, Kane A, Donnadieu AC, Levy-Zauberman Y, Vernay T, Hugueny J, Vincens E, Louis-Sylvestre C, Subtil A, Wu Y (2021) Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile. Sci Rep 11: 5848
- Hamaoui, D, Cossé, M.M., Mohan, J. Lystad, A.H., Wollert, T. and Subtil, A (2020). The Chlamydia effector CT622/TaiP targets a non-autophagy related function of ATG16L1 PNAS 117(43):26784-26794 doi:10.1073/pnas.2005389117
- Maffei, B., Laverriere, M., Wu, Y., Triboulet, S., Perrinet, S., Duchateau, M., Matondo, M., Hollis, R. L., Gourley, C., Rupp, J., Keillor, J. W., and Subtil, A. (2020) Infection-driven activation of transglutaminase 2 boosts glucose uptake and hexosaminebiosynthesis in epithelial cells. Embo J. 39(8):e102166
- Cossé M, Barta ML, Fiser DJ, Oesterlin LK, Niragire B, Perrinet S, Millot GA, Hefty PS, Subtil A The loss of expression of a single type 3 effector (CT622) strongly reduces Chlamydia trachomatis infectivity and growth Front Cell Infect Microbiol. 2018 8:145
Dernières publications sur HAL :
- [hal-04179689] Observation and Interview-based Diurnal Sleepiness Inventory for measurement of sleepiness in patients referred...by ano.nymous@ccsd.cnrs.fr.invalid (Laure Peter-Derex) on 10 August 2023 at 11h06
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- [hal-03869457] PI4P and BLOC-1 remodel endosomal membranes into tubulesby ano.nymous@ccsd.cnrs.fr.invalid (Riddhi Atul Jani) on 24 November 2022 at 12h03
The endosomal sorting complexes required for transport (ESCRT) system is an ancient and ubiquitous membrane scission machinery that catalyzes the […]
- [pasteur-03773446] CT295 Is Chlamydia trachomatis’ Phosphoglucomutase and a Type 3 Secretion Substrateby ano.nymous@ccsd.cnrs.fr.invalid (Sébastien Triboulet) on 9 September 2022 at 11h11
The obligate intracellular bacteria Chlamydia trachomatis store glycogen in the lumen of the vacuoles in which they grow. Glycogen catabolism […]
- [hal-03757326] Chlamydia trachomatis development requires both host glycolysis and oxidative phosphorylation but has only minor...by ano.nymous@ccsd.cnrs.fr.invalid (Maimouna D N’gadjaga) on 22 August 2022 at 15h03
The obligate intracellular bacteria Chlamydia trachomatis obtain all nutrients from the cytoplasm of their epithelial host cells and stimulate […]