Sandra Duharcourt
Institut Jacques Monod, 15 rue Hélène Brion 75013 Paris
Epigenetic regulation of genome organization
Website: https://www.ijm.fr/research-topics/research-groups/duharcourt-lab-va/?lang=en
ORCID: 0000-0002-8913-8799
Twitter: @DuharcourtLab
Our work aims to understand the fundamental mechanisms governing chromosome structure and genetic stability in eukaryotes. We study a remarkable process of genome editing that occurs during development in the unicellular eukaryote Paramecium.
Keywords: epigenetics, transposable elements, non-coding RNA, chromatin, Polycomb, genome evolution, Paramecium
Top 5 publications
1.Miró-Pina C, Charmant O, Kawaguchi T, Holoch D, Michaud A, Cohen I, Humbert A, Jaszczyszyn Y, Chevreux G, Del Maestro L, Ait-Si-Ali S, Arnaiz O, Margueron R, Duharcourt S. (2022) Paramecium Polycomb Repressive Complex 2 physically interacts with the small RNA binding PIWI protein to repress transposable elements. Dev Cell. 2022 Apr 6;S1534-5807(22)00208-8. doi: 10.1016/j.devcel.2022.03.014. PMID: 35429435
2.Gnan S#, Matelot M#, Weiman M, Arnaiz O, Guérin F, Sperling L, Bétermier M, Thermes C, Chen CL*, Duharcourt S*. (2022) GC content but not nucleosome positioning directly contributes to intron-splicing efficiency in Paramecium. Genome Res. 2022 Mar 9;gr.276125.121. doi: 10.1101/gr.276125.121. PMID: 35264448
3. Sellis S#, Guérin F#, Arnaiz O, Pett W, Lerat E, Boggetto N, Krenek S, Berendonk T, Couloux A, Aury J-M, Labadie K, Malinsky S, Bhullar S, Meyer E, Sperling L, Duret L*, Duharcourt S*. (2021). Massive colonization of protein-coding exons by selfish genetic elements in Paramecium germline genomes. PLoS Biol 19(7): e3001309. https://doi.org/10.1371/journal.pbio.3001309 PMID: 34324490
4. Vanssay A#, Touzeau A#, Arnaiz O, Frapporti A, Phipps J, Duharcourt S. (2020). The Parameciumhistone chaperone Spt16-1 is required for Pgm endonuclease function in programmed genome rearrangements. PLoS Genet. 2020 Jul 23;16(7):e1008949. doi: 10.1371/journal.pgen.1008949. PMID: 32702045
5. Frapporti A#, Miró-Pina C#, Arnaiz O, Holoch D, Kawaguchi T, Humbert A, Eleftheriou E, Lombard B, Loew D, Sperling L, Guitot K, Margueron R, Duharcourt S. (2019). The Polycomb protein Ezl1 mediates H3K9 and H3K27 methylation to repress transposable elements in Paramecium. Nat Commun. 2019 Jun 20;10(1):2710. doi: 10.1038/s41467-019-10648-5. PMID: 31221974
Dernières publications sur HAL :
- [hal-04239563] The nuclear PIWI-interacting protein Gtsf1 controls the selective degradation of small RNAs in Parameciumby ano.nymous@ccsd.cnrs.fr.invalid (Olivia Charmant) on 12 October 2023 at 17h04
Abstract Ciliates undergo developmentally programmed genome elimination, in which small RNAs direct the removal of target DNA segments, including […]
- [hal-04239559] Non-catalytic function of PRC2 in the control of small RNA dynamics during programmed genome elimination in...by ano.nymous@ccsd.cnrs.fr.invalid (Caridad Miró-Pina) on 12 October 2023 at 17h03
Abstract To limit transposable element (TE) mobilization, most eukaryotes have evolved small RNAs to silence TE activity via homology-dependent […]
- [hal-04126944] DNAModAnnot: a R toolbox for DNA modification filtering and annotationby ano.nymous@ccsd.cnrs.fr.invalid (Alexis Hardy) on 13 June 2023 at 14h46
Abstract Motivation Long-read sequencing technologies can be employed to detect and map DNA modifications at the nucleotide resolution on a […]
- [hal-03985655] The Polycomb protein Ezl1 mediates H3K9 and H3K27 methylation to repress transposable elements in Parameciumby ano.nymous@ccsd.cnrs.fr.invalid (Andrea Frapporti) on 13 February 2023 at 12h33
Abstract In animals and plants, the H3K9me3 and H3K27me3 chromatin silencing marks are deposited by different protein machineries. H3K9me3 is […]