Florence Niedergang

Website: https://institutcochin.fr/en/equipes/biology-phagocytes-infection-and-immunity
ORCID: 0000-0002-7064-830X
Twitter: @FNiedergang

Our team has a long-standing interest in professional phagocytes that play a major role in innate and adaptive immune responses. Phagocytosis, the mechanism of capture and degradation of invading microorganisms or debris, is crucial for homeostasis, bacterial clearance and to control the outcome of immune responses. Therefore, it is crucial to understand the mechanisms of phagocytosis, especially of cell debris or microorganisms.

We dissect the mechanisms used by phagocytes, in particular the coordinated activities of signaling pathways, membrane trafficking and cytoskeleton dynamics, and their related mechanical constraints, in order to mediate uptake of particulate material. We are also investigating the related cell activation and the fate of the internalized materials, from the mechanisms of their degradation to their release as non-degraded material that can be recognized by the B lymphocytes.

Additionally, we are deeply interested in understanding how viruses, including HIV-1, Zika virus and respiratory viruses such as the Human Rhinovirus and SARS-Cov2, impair the clearance and activation functions of phagocytes, leading to associated pathologies such as neuronal damage or bacterial superinfections, with a special focus on the role of the type I interferon response.

MATERIAL

• Phagocytosis experiments
• Phagocytic cells isolation, culture, manipulation (mouse, human, macrophages, dendritic cells, microglial cells, etc)
• Live cell imaging of infecrted cells (spinning disk microscope in a BSL3)
• NFkB and type I IFN pathways analysis

Top 5 publications

• Gatineau, J, Nidercorne, C, Dupont, A, Marie-LinePuiffe, M-L, Cohen, JL,Molinier-Frenkel, V*, Niedergang, F* and Castellano, F*. IL4I1 binds to TMPRSS13 and competes with SARS-Cov2 Spike. bioRxiv (2021) doi: https://doi.org/10.1101/2021.12.23.473686. Front Immunol. 2022 Sep 5;13:982839. doi: 10.3389/fimmu. 2022.9 82839. Article (* co-last authors)
• Jubrail J, Africano-Gomez K, Herit F, Mularski A, Bourdoncle P, Oberg L, Israelsson E, Burgel PR, Mayer G, M Cunoosamy D, Kurian N and Niedergang   Arpin is critical for phagocytosis in macrophages and is targeted by human rhinovirus. EMBO Reports (2020), 21, e47963. doi: 10.15252/embr.201947963. Article
• El-Barbry, H., Capitao, M., Barrin, S., Amziani, S., Pierre-Paul, P., Borreill, S., Guilbert, T., Donnadieu, E., Niedergang, F*, and Ouaaz, F*. Extracellular release of antigen by dendritic cell regurgitation promotes B cell activation through NF-κB/cRel. The Journal of Immunology (2020), 205:608-618. Article (* co-last authors)
• Boncompain G, Herit F, Tessier S, Lescure A, Del Nery E, Gestraud P, Staropoli, I, Fukuta Y, Fukuta M , Brelot A, Niedergang F, Perez F. Differential screening identifies molecules specifically inhibiting CCR5 transport to the cell surface and HIV infection. Doi.org/10.101364927. Science Advances (2019), eaax0821. Article
• Niedergang F and Grinstein S. How to build a phagosome: new concepts for an old process. Current Opinion in Cell Biology (2018) 50:57-63. Review

Dernières publications sur HAL :