Pierre Buffet

Website: https://sites.google.com/view/umrs1134-bigr/accueil
ORCID:0000-0003-4010-929X

The spleen, a major immune organ, contributes to the circulation of red blood cells, a process essential for survival. Rigid or adherent red blood cells are retained and eliminated by the spleen to prevent inadequate blood flow in other organs. The human spleen controls the quality of red blood cells by stringently challenging their deformability.
We study the circulatory capacity of red blood cells with reference tools (histology, cell morphology, ektacytometry, interference contrast, electron microscopy, mouse model, etc.) and with innovative methods (filtration by microbeads, flow imaging, ex-vivo perfusion of human spleens, splenomimetic microfluidics …). We have defined the splenic filtering functional unit, the splenon, by analogy with the renal filtering unit, the nephron. We also study the spleen-specific pitting process of pitting, by which rigid bodies are expelled from the red blood cell that returns to the circulation almost intact.
Concepts and applications are explored and envisioned in malaria, transfusion and red cell diseases.

Top 5 publications

1. Guery R, Walker SL, Harms G, Neumayr A, Van Thiel P, Gangneux JP, Clerinx J, Karlsson Söbirk S, Visser L, Lachaud L, Bailey M, Bart A, Ravel C, Van der Auwera G, Blum J, Lockwood DN, Buffet P with the LeishMan Network and the French Cutaneous Leishmaniasis Study group. Clinical diversity and treatment results in Tegumentary Leishmaniasis: A European clinical report in 459 patients. PloS NTD 2021;15 10: e0009863
2. RousselC, NdourPA, Kendjo E, Larréché S, Taieb A,Henry B, Lebrun-Vignes B,Chambrion C, Argy, Houzé S, Mouri O, Courtin D, Angoulvant A, Delacour H, Gay F, Siriez JY, Danis M, Bruneel F, Bouchaud O, Caumes E, Piarroux R, Thellier M, Jauréguiberry S*, Buffet P*. Intravenous artesunate for the treatment of severe imported malaria: implementation, efficacy and safety in 1391 patients.Clin Inf Dis 2021, DOI:1093/cid/ciab13
3. Kho S, Qotrunnada L, Leonardo L, Andries B, Wardani PAI, Fricot A, Henry B, Hardy D, Margyaningsih NI, Apriyanti D, Puspitasari AM, Prayoga P, Trianty L, Kenangalem E, Chretien F, Safeukui I, Del Portillo HA, Fernandez-Becerra C, Meibalan E, Marti M, Price RN, Woodberry T, Ndour PA, Russell BM, Yeo TW, Minigo G, Noviyanti R, Poespoprodjo JR, Siregar NC, Buffet PA*, Anstey NM*. Hidden Biomass of Intact Malaria Parasites in the Human Spleen. N Engl J Med 2021, 384, 2067-2069. 1056/NEJMc2023884       * Equal contributions
4. Henry B, Volle G, Akpovi H, Gineau L, Roussel C, Ndour PA, Tossou F, Suarez F, Palstra F, Fricot A, Chambrion C, Solinc J, Nguyen J, Garé M, Aussenac F, Cottart CH, Keyser C, Adamou R, Tichit M, Hardy D, Fievet N, Clain J, Garcia A, Courtin D, Hermine O, Sabbagh A, Buffet P. Splenic clearance of rigid erythrocytes as an inherited mechanism for splenomegaly and natural resistance to malaria. EBioMedicine 2022, 82, 104167 1016/j.ebiom.2022.104167
5. Carucci M, Duez J, Tarning J, García-Barbazán I, Fricot-Monsinjon A, Sissoko A, Dumas L, Gamallo P, Beher B, Amireault P, Dussiot M, Dao M, Hull M, McNamara C, Roussel C, Ndour PA, Sanz LM, Gamo FJ, Buffet P.^. Safe drugs with high potential to block malaria transmission revealed by a spleen mimetic screening. Nature Communications 2023, org/10.1038/s41467-023-37359-2

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