Florence Chapeland-Leclerc

Website: http://www.lied-pieri.univ-paris-diderot.fr/
ORCID: 0000-0002-6672-5501

The LIED is an interdisciplinary laboratory that brings together some twenty teacher-researchers from three main disciplines: Biology, Physics and Human and Social Sciences. Each teacher-researcher of the LIED belongs to a disciplinary component and participates, according to his or her discipline, in one or more interdisciplinary investigations. Thus, the general objective of LIED is to provide an interdisciplinary response to the energy issue. The four biology teams of the LIED are organised around a central theme “Living Systems Processes”. They are mainly interested in the analysis of organisms involved in carbon cycle ecosystems, in particular filamentous fungi which are the organisms that recycle and degrade biomass.

In this context, the central theme of the B2C team’s research activities, which I have been leading since 2016, is to better understand how fungi interact with their competitive environment and, in particular, what their involvement in soil health is by monitoring various physiological properties. To this end, two main research axes were initiated when the team was created. On the one hand, we seek to characterise the secondary metabolites produced by the fungi and, on the other hand, to better understand the growth dynamics of the mycelial network. Our research is carried out in particular on the model filamentous saprophyte fungus P. anserina, whose development cycle is perfectly mastered in the laboratory and which offers a wide range of useful genetic and cytological tools.

III-1-1 Fungal secondary metabolites

These are small molecules with great chemical diversity that allow organisms to adapt better to their environment. For humans, fungi represent an unlimited and still little-known source of bioactive metabolites that can be used in therapy. In this context, our first and fundamental objective is to better understand the role of these metabolites in the life cycle of P. anserina. The second objective, more applied, is to explore the genetic regulation and diversity of secondary metabolites in P. anserina in order to identify new molecules and to evaluate their potential interest, particularly in therapeutics.

Collaborations : Biologists at LIED (B2C team) and chemists: Natural Product, Analysis and Synthesis team (PNAS, Université Paris Cité; T. Gaslonde and X. Cachet), Chimiodiversity of Marine Mushrooms and Valorisations team (ChiChaMVa, Université de Nantes; C. Roullier and O. Grovel), the Organic Chemistry and Interfaces team (COrInt, University of Rennes; FH Porée) and the Chemistry of Natural Fungal Products team (CPNF, Muséum National d’Histoire Naturelle; S. Prado). Within the Université Paris Cité, the AAP IdEx Émergence (2020) has enabled us to consolidate our relations with the chemists of the Pharmacy site. We must now continue our collaboration with more long-term structuring projects (ANR in particular).

Publications related to this theme: (2), (3) and (5).

III-1-2 Mycelial network

An original characteristic of filamentous fungi is their organisation in a network of hyphae with the possibility of branching and fusing hyphae. The network allows, among other things, optimal exploration and exploitation of the growth medium, in particular by allowing the passage through unfavourable areas. Our objective is therefore to analyse the structure and growth dynamics of the mycelial network of P. anserina subjected to external constraints with a view to developing a predictive model. For this purpose, quantitative measurement tools at the network level have been developed, in particular for the detection of nodes (branches), apexes and the total length of the network. This approach now provides us with quantitative tools to test the effect of growth conditions (i.e. the environment) on the mycelial network and its organisation.

This line of research is carried out by an interdisciplinary association at the LIED between biologists (F. Chapeland-leclerc, G. Ruprich-Robert and E. Cabet: B2C team), physicists (E. Herbert, F. Filaine and P. David), a biostatistician (C. Lalanne), a CNRS/LIED engineer in geomatics (C. Bobée), associated with mathematicians: Laboratoire JA Dieudonné (LJAD, Université de Nice Sophia Antipolis; Y. d’Angelo, R. Catellier, C. Guerrier and L. Monasse), Centre de Mathématiques Appliquées (CMAP, Ecole Polytechnique; M. Tomasevic) and Département de Mathématiques Appliquées (MAP5, Université Paris Cité; A. Veber).

We will also take advantage of the arrival at LIED of C. Villard (DR CNRS, physicist), who is interested in the behaviour of Candida albicans hyphae under different constraints, to develop an approach in microfluidics, a field in which she is an expert. In addition, very promising exchanges are underway with the Institut Français du Pétrole et des Energies Nouvelles (IFPEN) for an effective collaboration in this research area. Finally, the award of an IdEx Emergence in 2019 followed by an ANR in 2021 have contributed greatly to the structuring of the consortium.

Top 5 publications

(1). Ledoux C., Chapeland-Leclerc F., Ruprich-Robert G., Bobée C., Lalanne C., Herbert E. and David P. (2022). Prediction and experimental evidence of the optimisation of the angular branching process in the thallus growth of Podospora anserina. Sci Rep. 12:12351 (IF=4,1).

(2). Shen L., Chapeland-Leclerc F., Ruprich-Robert G., Chena Q., Chena S., Adnana M., Wangd J., Liua G. and Xie N. (2022). Involvement of VIVID in white light‐responsive pigmentation, sexual development and sterigmatocystin biosynthesis in the filamentous fungus Podospora anserina. Environ Microbiol. Early view, online. (IF=5,5).

 (3). Shen L., Gaslonde T., Roullier C., Wang H., Bodin J., Porée F.-H., Ruprich-Robert G*., Chapeland-Leclerc F.* (*The two last authors contributed equally to this work). (2022). Functional characterization of the GATA-type transcription factor PaNsdD in the filamentous fungus Podospora anserina and its interplay with the sterigmatocystin pathway. Appl Environ Microbiol. 88:e0237821. (IF=4,8).

(4). Dikec J., Olivier A., Bobée C., D’Angelo Y., Catellier R., David P., Filaine F., Herbert S., Lalanne Ch., Lalucque H., Monasse L., Rieu M., Ruprich-Robert G., Véber A., Chapeland-Leclerc F.† Herbert E.† (†The two last authors contributed equally to this work). (2020). Hyphal network whole field imaging allows for accurate estimation of anastomosis rates and branching dynamics of the filamentous fungus Podospora anserina. Sci Rep. 10:3131. (IF=4,1).

(5). Shen L., Porée F.H., Gaslonde T., Lalucque H., Chapeland-Leclerc F.#, Ruprich-Robert G.# (#The two last authors contributed equally to this work). (2019). Functional characterization of the sterigmatocystin secondary metabolite cluster in the filamentous fungus Podospora anserina: involvement in oxidative stress response, sexual development, pigmentation and interspecific competitions. Environ Microbiol. 21:3011-3026 (IF=4,9).

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