Agathe Subtil

Website: https://research.pasteur.fr/en/team/cellular-biology-of-microbial-infection/
ORCID: 0000-0002-7481-4846

The goal of the work of the lab is to gain knowledge on the normal behavior of the eukaryotic cell, and on its disruptions during an infectious process. We focus on the mechanisms of entry and intracellular fate of intracellular bacteria, Chlamydia. Chlamydiae species pathogenic to humans, mainly Chlamydia trachomatis and Chlamydia pneumoniae, cause a number of diseases, including trachoma, pelvic inflammatory disease and pneumonia. Primary infections are often minor or asymptomatic; the consequences appear long after infection. Throughout their cycle in the host cell, chlamydiae remain in a membrane-bound compartment called an inclusion. We investigate the interactions between the bacteria and the cells during infection. We focus mainly on the functional study of proteins secreted by the bacteria into the host cytoplasm by a process called type III secretion. Recent themes of study include the interplay between the metabolism of the host and that of the pathogen, as well as the consequence of the infection on the epigenome of the host.

Top 5 publications

1. Triboulet S, N’Gadjaga M.D., Niragire B., Köstlbacher S., Horn M., Aimanianda V. and Subtil A. (2022) CT295 Is Chlamydia trachomatis’ Phosphoglucomutase and a Type 3 Secretion Substrate. Front Cell Inf Microbiol 12. doi 10.3389/fcimb.2022.866729
2. Tang C, Liu C, Maffei B, Niragire B, Cohen H, Kane A, Donnadieu AC, Levy-Zauberman Y, Vernay T, Hugueny J, Vincens E, Louis-Sylvestre C, Subtil A, Wu Y (2021) Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile. Sci Rep 11: 5848
3. Hamaoui, D, Cossé, M.M., Mohan, J. Lystad, A.H., Wollert, T. and Subtil, A (2020). The Chlamydia effector CT622/TaiP targets a non-autophagy related function of ATG16L1 PNAS 117(43):26784-26794 doi:10.1073/pnas.2005389117
4. Maffei, B., Laverriere, M., Wu, Y., Triboulet, S., Perrinet, S., Duchateau, M., Matondo, M., Hollis, R. L., Gourley, C., Rupp, J., Keillor, J. W., and Subtil, A. (2020) Infection-driven activation of transglutaminase 2 boosts glucose uptake and hexosaminebiosynthesis in epithelial cells. Embo J. 39(8):e102166
5. Cossé M, Barta ML, Fiser DJ, Oesterlin LK, Niragire B, Perrinet S, Millot GA, Hefty PS, Subtil A The loss of expression of a single type 3 effector (CT622) strongly reduces Chlamydia trachomatis infectivity and growth Front Cell Infect Microbiol. 2018 8:145

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