Philippe Bastin

Website: https://research.pasteur.fr/en/team/trypanosome-cell-biology/
ORCID: 0000-0002-3042-8679
Twitter: @BastinLab_Paris

The main projects of the Bastin lab aim to understand (1) how intraflagellar transport (IFT) is initiated from protein complexes to moving trains in the flagellum and (2) how diversity can be generated between different types of cilia and flagella but also within the organelle based on the tubulin code, especially the post-translational addition of glutamates to the tail of tubulins. For its construction, the flagellum relies on a sophisticated process of intraflagellar transport. Initially, the group had identified and characterised multiple molecular components involved in the pathway identified either by homology or by proteomics following trypanosome flagella purification. New concepts have been proposed in terms of IFT train distribution and flagellum length control to investigate how these actors are put in action, how they are modulated to produce different types of flagella and how this knowledge can be exploited to understand ciliopathies. The fast (2-5 µm/sec) movement on a constrained space (9 doublets microtubules in a cylinder of 180 nm in diameter) drove them to develop challenging state-of-the art imaging approaches to decipher the dynamics of the IFT process. They pursue investment in innovative approaches such as single cell analysis, super-resolution microscopy, expansion microscopy, electron microscopy, biochemistry, and various reverse genetics approaches.

Top 5 publications

1- Hutchinson, S., Foulon, S., Crouzols, A., Menafra, R., Rotureau, B., Griffiths, A.D., and Bastin, P. (2021).
The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands.
PLoS Pathog 17, e1009904. 10.1371/journal.ppat.1009904.

2- Calvo-Alvarez, E., Bonnefoy, S., Salles, A., Benson, F.E., McKean, P.G., Bastin, P., and Rotureau, B. (2021).
Redistribution of FLAgellar Member 8 during the trypanosome life cycle: Consequences for cell fate prediction.
Cell Microbiol 23, e13347. 10.1111/cmi.13347.

3- Atkins, M., Tyc, J., Shafiq, S., Ahmed, M., Bertiaux, E., De Castro Neto, A.L., Sunter, J., Bastin, P., Dean, S.D., and Vaughan, S. (2021).
CEP164C regulates flagellum length in stable flagella.
J Cell Biol 220. 10.1083/jcb.202001160.

4- Bonnefoy, S., Watson, C.M., Kernohan, K.D., Lemos, M., Hutchinson, S., Poulter, J.A., Crinnion, L.A., Berry, I., Simmonds, J., Vasudevan, P., O’Callaghan, C., Hirst, R.A., Rutman, A., Huang, L., Hartley, T., Grynspan, D., Moya, E., Li, C., Carr, I.M., Bonthron, D.T., Leroux, M., Care4Rare Canada, C., Boycott, K.M., Bastin, P., and Sheridan, E.G. (2018).
Biallelic Mutations in LRRC56, Encoding a Protein Associated with Intraflagellar Transport, Cause Mucociliary Clearance and Laterality Defects.
Am J Hum Genet 103, 727-739.

5- Bertiaux, E., Morga, B., Blisnick, T., Rotureau, B., and Bastin, P. (2018).
A Grow-and-Lock Model for the Control of Flagellum Length in Trypanosomes.
Curr Biol 28, 3802-3814 e3803.

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